Michael C. Dovey, Leonard I. Zon (auth.), John S. Yu (eds.)'s Cancer Stem Cells: Methods and Protocols PDF

By Michael C. Dovey, Leonard I. Zon (auth.), John S. Yu (eds.)

ISBN-10: 1588299384

ISBN-13: 9781588299383

Through the innovative thought of melanoma stem cells, melanoma examine has been reinvigorated to check the function of those distinctive cells in melanoma propagation and as pursuits of cutting edge cures. In Cancer Stem Cells: tools and Protocols, preeminent researchers have compiled melanoma stem telephone examine options and protocols to advertise fit festival, discourse, and collaboration during this important box. the quantity covers broad subject matters equivalent to id and isolation of melanoma stem cells, animal types of melanoma stem cells, methylation profiling, the contribution of the area of interest within the legislation of melanoma stem cells, immunologic concentrating on, and using general stem cells as a remedy, between different matters. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters contain short introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, without difficulty reproducible laboratory protocols, and specialist notes on troubleshooting and keeping off identified pitfalls.

Comprehensive and state of the art, Cancer Stem Cells: equipment and Protocols will relief scientists around the globe within the furthering of our realizing of melanoma initiation and propagation and, most significantly, within the improvement of novel goals for melanoma therapy.

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Mouse Irradiation 1. Irradiate the syngenic or congenic mice (8–12 weeks old) in which the leukemic cells are to be injected at lethal doses of radiation (800–850 cGy of whole body irradiation) (see Note 11) ∼2–3 h prior to injection. 4. Injection of Mice 1. Transfer the lethally irradiated mouse from the cage and place it gently into a mouse-restraint device exposing the tail. 2. Prewarm the tail of the restrained mouse for vasodilation with a safe heat source such as warm water or heat lamp for 30–60 s (see Note 12).

Transplanted mice develop signs of disease at variable time points (4–12 weeks). Inspect mice regularly for morbidity. Euthanize moribund mice with CO2 asphyxiation and dissect out the hind limb and pelvic bones. If necessary BM aspirations may be performed to detect leukemic engraftment at time points prior to the termination of mice. 4. BM Aspiration by Femur Punctures BM aspirations may be performed on anesthetized mice to detect leukemic engraftment at 3-week intervals starting from week 3 post-transplantation.

E. A. McCulloch (1977), Colony assay for proliferative blast cells circulating in myeloblastic leukaemia. Lancet. 1(8016): p. 862–3. 13. , et al. (1991), Differential regulation of primitive human hematopoietic 14. 15. 16. 17. 18. 35 cells in long-term cultures maintained on genetically engineered murine stromal cells. Blood. 78(3): p. 666–72. , et al. (1999), Growth characteristics of acute myelogenous leukemia progenitors that initiate malignant hematopoiesis in nonobese diabetic/severe combined immunodeficient mice.

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Cancer Stem Cells: Methods and Protocols by Michael C. Dovey, Leonard I. Zon (auth.), John S. Yu (eds.)

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